Cancer Diagnostics with DNA Microarrays

Science
Cancer Diagnostics with DNA Microarrays PDF Author: Steen Knudsen
Publisher: John Wiley & Sons
ISBN: 0470041099
Category : Science
Languages : en
Pages : 272

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Book Description
Authored by an international authority in the field,Cancer Diagnostics with DNA Microarrays is a complete reference work on the rapidly growing use of DNA microarray data in the diagnosis of and treatment planning for a large number of human cancers. Uniquely deals with direct clinical application of microarray data to oncology diagnosis, leading to more effective diagnosis of and clearer treatment regimens for a wide range of human cancers Offers clinicians summary presentation of state-of-the-art science of DNA microarrays Each chapter includes bibliographic and further reading suggestions Easily accessible, assuming no special training in statistics or bioinformatics Replete with examples and mini-cases, Cancer Diagnostics with DNA Microarrays offers cancer researchers in private, pharmacologic, and governmental institutions, biomedical statisticians, and practicing oncologists concise, thoughtfully authored guidance on the use of microarray data and analysis as clinical tools. The text carefully addresses the needs of end users – researchers and physicians – using microarrays as a tool to be applied in common clinical situations, and is of interest for students in medicine and biology and professionals in health care as well.

Cancer Diagnostics with DNA Microarrays

Cancer Diagnostics with DNA Microarrays PDF Author: Steen Knudsen
Publisher: John Wiley & Sons
ISBN: 0470041099
Category : Science
Languages : en
Pages : 272

View

Book Description
Authored by an international authority in the field,Cancer Diagnostics with DNA Microarrays is a complete reference work on the rapidly growing use of DNA microarray data in the diagnosis of and treatment planning for a large number of human cancers. Uniquely deals with direct clinical application of microarray data to oncology diagnosis, leading to more effective diagnosis of and clearer treatment regimens for a wide range of human cancers Offers clinicians summary presentation of state-of-the-art science of DNA microarrays Each chapter includes bibliographic and further reading suggestions Easily accessible, assuming no special training in statistics or bioinformatics Replete with examples and mini-cases, Cancer Diagnostics with DNA Microarrays offers cancer researchers in private, pharmacologic, and governmental institutions, biomedical statisticians, and practicing oncologists concise, thoughtfully authored guidance on the use of microarray data and analysis as clinical tools. The text carefully addresses the needs of end users – researchers and physicians – using microarrays as a tool to be applied in common clinical situations, and is of interest for students in medicine and biology and professionals in health care as well.

DNA Microarray Technology and Data Analysis in Cancer Research

DNA Microarray Technology and Data Analysis in Cancer Research PDF Author: Shaoguang Li
Publisher: World Scientific
ISBN: 9812790454
Category : Medical
Languages : en
Pages : 118

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Book Description
DNA microarray technology has become a useful technique in gene expression analysis for the development of new diagnostic tools and for the identification of disease genes and therapeutic targets for human cancers. Appropriate control for DNA microarray experiment and reliable analysis of the array data are key to performing the assay and utilizing the data correctly. The most difficult challenge has been the lack of a powerful method to analyze the data for all genes (more than 30,000 genes) simultaneously and to use the microarray data in a decision-making process. In this book, the authors describe DNA microarray technology and data analysis by pointing out current advantages and disadvantages of the technique and available analytical methods. Crucially, new ideas and analytical methods based on the authors' own experience in DNA microarray study and analysis are introduced. It is believed that this new way of interpreting and analyzing microarray data will bring us closer to success in decision-making using the information obtained through the DNA microarray technology.

Expression Profiling of Human Tumors

Expression Profiling of Human Tumors PDF Author: Marc Ladanyi
Publisher: Springer Science & Business Media
ISBN: 1592593860
Category : Medical
Languages : en
Pages : 399

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Book Description
A comprehensive review of the use of global gene expression profiling to understand human tumors. The authors focus on the analysis of human tissue samples for a variety of cancers, including breast, colorectal, lung, renal, ovarian, bone, and brain tumors, among others. A primer on the technology and its novel analytical methods. Contains over 40 color images. Explains both the theory and practice of comprehensive gene expression profiling. Timely and topical, Expression Profiling of Human Tumors: Diagnostic and Research Applications offers every oncologist, pathologists, and cancer surgeons an essential introduction to the most promising new high-throughput investigative approaches in molecular biology-technology that is already dramatically reshaping the future of cancer research, diagnostic pathology, and clinical oncology.

Microarrays in Diagnostics and Biomarker Development

Microarrays in Diagnostics and Biomarker Development PDF Author: Bertrand Jordan
Publisher: Springer Science & Business Media
ISBN: 3642282032
Category : Medical
Languages : en
Pages : 240

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Book Description
Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards “nanoarrays”, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.

Developing Biomarker-Based Tools for Cancer Screening, Diagnosis, and Treatment

Developing Biomarker-Based Tools for Cancer Screening, Diagnosis, and Treatment PDF Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309101344
Category : Medical
Languages : en
Pages : 112

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Book Description
Research has long sought to identify biomarkers that could detect cancer at an early stage, or predict the optimal cancer therapy for specific patients. Fueling interest in this research are recent technological advances in genomics, proteomics, and metabolomics that can enable researchers to capture the molecular fingerprints of specific cancers and fine-tune their classification according to the molecular defects they harbor. The discovery and development of new markers of cancer could potentially improve cancer screening, diagnosis, and treatment. Given the potential impact cancer biomarkers could have on the cost effectiveness of cancer detection and treatment, they could profoundly alter the economic burden of cancer as well. Despite the promise of cancer biomarkers, few biomarker-based cancer tests have entered the market, and the translation of research findings on cancer biomarkers into clinically useful tests seems to be lagging. This is perhaps not surprising given the technical, financial, regulatory, and social challenges linked to the discovery, development, validation, and incorporation of biomarker tests into clinical practice. To explore those challenges and ways to overcome them, the National Cancer Policy Forum held the conference "Developing Biomarker-Based Tools for Cancer Screening, Diagnosis and Treatment: The State of the Science, Evaluation, Implementation, and Economics" in Washington, D.C., from March 20 to 22, 2006. At this conference, experts gave presentations in one of six sessions. In addition, seven small group discussions explored the policy implications surrounding biomarker development and adoption into clinical practice. Developing Biomarker-based Tools for Developing Cancer Screening, Diagnosis, and Treatment: The State of the Science, Evaluation, Implementation, and Economics-Workshop Summary presents the conference proceedings and will be used by an Institute of Medicine (IOM) committee to develop consensus-based recommendations for moving the field of cancer biomarkers forward.

Microarray Technology in Practice

Microarray Technology in Practice PDF Author: Steve Russell
Publisher: Academic Press
ISBN: 9780080919768
Category : Science
Languages : en
Pages : 464

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Book Description
Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays

High-Throughput Array Instrument for DNA-Based Breast Cancer Diagnostics

High-Throughput Array Instrument for DNA-Based Breast Cancer Diagnostics PDF Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 12

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Book Description
The aim of this project is to develop improved methods for the production, processing, and analysis of microarrays (gene-chips) for breast-cancer diagnostics. These methods are based upon large numbers of discrete DNA spots placed on glass microscope slides typically, and hybridized to a probe derived from a tIssue or blood sample. %or breast- cancer diagnostics, there are two likely predominant modes of use. TIssue samples from suspect lesions, e.g., can be assessed for patterns of gene expression on cDNA arrays, indicative of a specific disease type or state. Additionally, analysis of known mutations can be performed on oligonucleotide-based arrays. We are optimizing cDNA microarray methods, and have achieved excellent limits of detection, although not good enough to detect the rarest transcripts. To improve this situation, we are redesigning our novel imaging (non-scanning) fluorescence microarray reader that currently performs four times better (with respect to limit of detection) than a commercial scanner, and does so about 300 times faster. Our next goal is to implement oligonucleotide arrays by various methods including both padlock probe-based ligation assays and allele-specific hybridization tests for specific known mutations. We are also actively improving direct covalent attachment of oligonucleotides to glass, and methods for printing oligonucleotide arrays much faster and cheaper.

Biosensor Based Advanced Cancer Diagnostics

Biosensor Based Advanced Cancer Diagnostics PDF Author: Raju Khan
Publisher: Academic Press
ISBN: 0128236515
Category : Technology & Engineering
Languages : en
Pages : 438

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Book Description
Early diagnosis of cancer and other non-oncological disorders gives a significant advantage for curing the disease and improving patient’s life expectancy. Recent advances in biosensor-based techniques which are designed for specific biomarkers can be exploited for early diagnosis of diseases. Biosensor Based Advanced Cancer Diagnostics covers all available biosensor-based approaches and comprehensive technologies; along with their application in diagnosis, prognosis and therapeutic management of various oncological disorders. Besides this, current challenges and future aspects of these diagnostic approaches have also been discussed. This book offers a view of recent advances and is also helpful for designing new biosensor-based technologies in the field of medical science, engineering and biomedical technology. Biosensor Based Advanced Cancer Diagnostics helps biomedical engineers, researchers, molecular biologists, oncologists and clinicians with the development of point of care devices for disease diagnostics and prognostics. It also provides information on developing user friendly, sensitive, stable, accurate, low cost and minimally invasive modalities which can be adopted from lab to clinics. This book covers in-depth knowledge of disease biomarkers that can be exploited for designing and development of a range of biosensors. The editors have summarized the potential cancer biomarkers and methodology for their detection, plus transferring the developed system to clinical application by miniaturization and required integration with microfluidic systems. Covers design and development of advanced platforms for rapid diagnosis of cancerous biomarkers Takes a multidisciplinary approach to sensitive transducers development, nano-enabled advanced imaging, miniaturized analytical systems, and device packaging for point-of-care applications Offers an insight into how to develop cost-effective diagnostics for early detection of cancer

Cancer Diagnostics

Cancer Diagnostics PDF Author: Robert M. Nakamura
Publisher: Springer Science & Business Media
ISBN: 1592597912
Category : Medical
Languages : en
Pages : 508

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Book Description
Reviews recent and emerging clinical laboratory tests that can help in the early detection, evaluation, and prediction of human tumors. Emphasizing the importance of molecular and genetic RNA/DNA tets that detect persons at high risk for specific cancers, the authors explore these novel serological assays, cellular assays useful for anatomic pathology, and molecular and genetic assays.

DNA Microarray Technology and Data Analysis in Cancer Research

DNA Microarray Technology and Data Analysis in Cancer Research PDF Author:
Publisher:
ISBN: 981447200X
Category :
Languages : en
Pages :

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Book Description