Microarrays and Cancer Research

Microarrays and Cancer Research PDF Author: Janet A. Warrington
Publisher: Eaton Publishing Company
ISBN:
Category : Medical
Languages : en
Pages : 162

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Cancer Diagnostics with DNA Microarrays

Cancer Diagnostics with DNA Microarrays PDF Author: Steen Knudsen
Publisher: John Wiley & Sons
ISBN: 0470041099
Category : Science
Languages : en
Pages : 272

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Book Description
Authored by an international authority in the field,Cancer Diagnostics with DNA Microarrays is a complete reference work on the rapidly growing use of DNA microarray data in the diagnosis of and treatment planning for a large number of human cancers. Uniquely deals with direct clinical application of microarray data to oncology diagnosis, leading to more effective diagnosis of and clearer treatment regimens for a wide range of human cancers Offers clinicians summary presentation of state-of-the-art science of DNA microarrays Each chapter includes bibliographic and further reading suggestions Easily accessible, assuming no special training in statistics or bioinformatics Replete with examples and mini-cases, Cancer Diagnostics with DNA Microarrays offers cancer researchers in private, pharmacologic, and governmental institutions, biomedical statisticians, and practicing oncologists concise, thoughtfully authored guidance on the use of microarray data and analysis as clinical tools. The text carefully addresses the needs of end users – researchers and physicians – using microarrays as a tool to be applied in common clinical situations, and is of interest for students in medicine and biology and professionals in health care as well.

Dna Microarrays As a Tool in Cancer Research

Dna Microarrays As a Tool in Cancer Research PDF Author: Antti Kokko
Publisher: LAP Lambert Academic Publishing
ISBN: 9783838318493
Category :
Languages : en
Pages : 88

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Book Description
DNA microarray technology has been rapidly adopted by biomedical researchers and emerged as a promising research method in studies of human cancer. In many cases in silico data alone is not sufficient to identify a cancer predisposing gene, but when used together with other methods such as linkage analysis and functional studies this becomes a very prominent tool. This study describes various methods used in microarray technology and how the technology was utilized in studies of certain hereditary cancers together with supporting methods and technologies. Although DNA microarrays are used primarily in research, clinical applications are foreseeable and slowly emerging. This study should give more insight to the potential of microarrays as a tool for cancer research. The book should be especially useful for undergraduate or graduate students in the field of molecular biology or cancer research who wish to know more about the microarray technology and how it may be used in conjunction with other methods in genetics, or for anyone else who may be interested in microarray experiments.

Microarrays in Cancer

Microarrays in Cancer PDF Author: Michael F. Ochs
Publisher:
ISBN:
Category :
Languages : en
Pages : 72

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Expression Profiling of Human Tumors

Expression Profiling of Human Tumors PDF Author: Marc Ladanyi
Publisher: Springer Science & Business Media
ISBN: 1592593860
Category : Medical
Languages : en
Pages : 399

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Book Description
A comprehensive review of the use of global gene expression profiling to understand human tumors. The authors focus on the analysis of human tissue samples for a variety of cancers, including breast, colorectal, lung, renal, ovarian, bone, and brain tumors, among others. A primer on the technology and its novel analytical methods. Contains over 40 color images. Explains both the theory and practice of comprehensive gene expression profiling. Timely and topical, Expression Profiling of Human Tumors: Diagnostic and Research Applications offers every oncologist, pathologists, and cancer surgeons an essential introduction to the most promising new high-throughput investigative approaches in molecular biology-technology that is already dramatically reshaping the future of cancer research, diagnostic pathology, and clinical oncology.

Cancer Genomics

Cancer Genomics PDF Author: Cécile Le Page
Publisher: Elsevier Inc. Chapters
ISBN: 0128061022
Category : Medical
Languages : en
Pages : 510

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Book Description
The tissue microarray (TMA) is a recent technology developed to increase the performance of molecular profiling in cancer research by rapidly performing large-scale studies while reducing experimental variables and conserving priceless tissue samples. The technology allows, on a single glass slide, the parallel analysis of protein or genes in an array composed of hundreds of paraffin-embedded cored tissue specimens. TMAs can be evaluated with immunohistochemistry, fluorescent-dye assays, and in situ hybridization (ISH). While strictly a research and screening tool, TMAs enable the rapid investigation of normal and cancer tissues, and are especially useful for cancer biomarker validation studies. In oncology, the ability to couple TMA results to clinical parameters is especially useful for the development of clinically useful prognostic and predictive biomarkers derived from genomics studies. This chapter will introduce the readers to the main technical aspects of TMA construction and the methods of TMA analysis particularly in the context of breast and ovarian cancers. Important points for consideration in order to avoid the common pitfalls of the technology will be presented and a particular focus will be provided on quality control and analysis of TMA.

Oncogenomics

Oncogenomics PDF Author: Charles Brenner
Publisher: John Wiley & Sons
ISBN: 0471476641
Category : Medical
Languages : en
Pages : 400

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Book Description
This book aims to assemble the only available rigorous, yet broadly accessible introduction to this new and exciting field. Oncogenomics: Molecular Approaches to Cancer is approachable by basic scientists, practitioners, and other health professionals required to familiarize themselves with the tremendous impact of genomics and proteomics on cancer research. Clearly written chapters offer reviews of state of the art topics such as molecular classification, early detection, SNPs in cancer, data mining, tissue microarrays, protein and antibody arrays, and drug targets.

Design and Analysis of DNA Microarray Investigations

Design and Analysis of DNA Microarray Investigations PDF Author: Richard M. Simon
Publisher: Springer Science & Business Media
ISBN: 0387218661
Category : Medical
Languages : en
Pages : 200

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Book Description
The analysis of gene expression profile data from DNA micorarray studies are discussed in this book. It provides a review of available methods and presents it in a manner that is intelligible to biologists. It offers an understanding of the design and analysis of experiments utilizing microarrays to benefit scientists. It includes an Appendix tutorial on the use of BRB-ArrayTools and step by step analyses of several major datasets using this software which is available from the National Cancer Institute.

Modern Molecular Biology:

Modern Molecular Biology: PDF Author: Srinivasan Yegnasubramanian
Publisher: Springer Science & Business Media
ISBN: 0387697454
Category : Medical
Languages : en
Pages : 192

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Book Description
Molecular biology has rapidly advanced since the discovery of the basic flow of information in life, from DNA to RNA to proteins. While there are several important and interesting exceptions to this general flow of information, the importance of these biological macromolecules in dictating the phenotypic nature of living creatures in health and disease is paramount. In the last one and a half decades, and particularly after the completion of the Human Genome Project, there has been an explosion of technologies that allow the broad characterization of these macromolecules in physiology, and the perturbations to these macromolecules that occur in diseases such as cancer. In this volume, we will explore the modern approaches used to characterize these macromolecules in an unbiased, systematic way. Such technologies are rapidly advancing our knowledge of the coordinated and complicated changes that occur during carcinogenesis, and are providing vital information that, when correctly interpreted by biostatistical/bioinformatics analyses, can be exploited for the prevention, diagnosis, and treatment of human cancers. The purpose of this volume is to provide an overview of modern molecular biological approaches to unbiased discovery in cancer research. Advances in molecular biology allowing unbiased analysis of changes in cancer initiation and progression will be overviewed. These include the strategies employed in modern genomics, gene expression analysis, and proteomics.

Application of Protein and Small-molecule Microarrays to Study Stem Cell Differentiation, Cancer Growth, and Personalized Therapy

Application of Protein and Small-molecule Microarrays to Study Stem Cell Differentiation, Cancer Growth, and Personalized Therapy PDF Author: Dayu Teng
Publisher:
ISBN: 9781124262482
Category :
Languages : en
Pages : 85

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Book Description
The DNA microarray technology has contributed widely in biological studies. Recently, a method to detect the effects of extracellular matrix (ECM) proteins on cells using microarray was developed. In this dissertation, the microarray technology was applied to study the effects of ECM proteins on human mesenchymal stem cell (hMSC) differentiation, and to identify the best growth-supporting ECM conditions for glioblastoma, and to create a method for efficient small molecule drug screening. The importance of ECM on hMSCs is well known. Numerous studies have shown that the ECM deposed by specific types of cells could induce differentiation in hMSCs. However, clinical application of cell-deposited ECM could carry risks such as contamination, immune rejection and batch-to-batch variation. An artificial ECM could avoid these problems. In this dissertation, the effects of ECM proteins were screened on the hMSCs differentiation using the microarray technology. The myogenic and neurogenic differentiation profiles are reported. This information can contribute to the selection of optimal differentiation-inducing coating on the muscle or nerve tissue engineering scaffolds. Cell culture has always been a fundamental tool in cancer research. However, most primary cancer cells are very difficult to maintain. In this dissertation, ECM protein combinations were tested for their efficacy in supporting the growth of glioblastoma cells. Twelve best combinations were identified. The result may lead to successful maintenance of the primary cells of glioblastoma and other types of cancerous tumors. The results may yield larger quantities of the primary cancer cells, which are heretofore limited in supply, and thus facilitate the advancement of cancer research. Genetic marker-based personalized medicine has attracted much attention. However, our understanding of the genes is still limited. A direct drug efficacy screening on the diseased cells of an individual patient may complement the gene-based personalized medicine. In this study, a small-molecule drug microarray method is reported. This method is able to screen a large number of drugs and their combinations by using a very small mount of patients' diseased cells, making it possible to design personalized drug cocktails.